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Knowledgebase:Choosing sequencing parameters

I am planning a project that requires the use of NGS technologies. How do I know what read length and sequencing depth to use? And should I use paired-end sequencing?

This article on GenoHub has a table with a number of referenced suggestions for different NGS projects:

https://genohub.com/recommended-sequencing-coverage-by-application/

More details can be found here:

https://genohub.com/next-generation-sequencing-guide/

If you have more questions, please feel free to request a meeting with someone on the BTS.

Other info:

Study design for finding variants associated with a disease:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291790/

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