MTB CRISPRi-seq

I helped Mandy to download her data (SFTP .fastq files from public site at Crick). Some of the files are controls (~500MB) to check if the sgRNA libraries are comprehensive and unbiased while other files contain experimental data (~2GB). Mandy would like to get a quick look at the control files to see if the libraries are good. I will look at running MAGECK on HPC as a nextflow pipeline (docker container available).