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From 04/21/2019 to 05/20/2019

05/17/2019

11:08 AM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #82 (In Progress): Preliminary analysis of Proteome identification data
Thys Potgieter
11:04 AM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #82 (Resolved): Preliminary analysis of Proteome identification data
Differential abundance analysis of preliminary data, PCA and heatmap generation. Thys Potgieter
10:58 AM Infant stool metaproteome analysis Support #75 (Resolved): Taxonomic profiling using BLAST of identified proteins and peptides
Thys Potgieter

05/16/2019

10:52 AM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #81 (Resolved): Full run on ilifu of RNA seq pipeline
Full run started on ilifu.
Output dir:
/ceph/cbio/users/jambler/full_run
Execute script used:
/ceph/cbio/use...
Jon Ambler

05/15/2019

09:28 AM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #80 (Resolved): Renaming all files according to the experiments
Files uploaded and renamed successfully. Jon Ambler

05/14/2019

02:58 PM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #80 (In Progress): Renaming all files according to the experiments
To make sure files are named correctly, a testing set was created where the files contained their original names to m... Jon Ambler
12:18 PM Pathogen outbreak study - Pseudomonas single isolate WGS srst2 AMR and VFDB results transferred
NB: there were some differences in results between Tychus and srst2 which seems to be due to different bowtie2 parame... Katie Lennard
10:30 AM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #78 (Resolved): Proteomics raw data uploaded to Blackburn Lab researchdata
Thys Potgieter

05/13/2019

10:58 AM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #80 (Resolved): Renaming all files according to the experiments
Relebohile asked if the fastq files could be renamed according to the experimental conditions. Jon Ambler
09:09 AM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #79 (Resolved): Uploading new data from BGI
Jon Ambler
09:09 AM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #79: Uploading new data from BGI
Download completed, checksums passed.
Files moved to:
/ceph/cbio/users/jambler/Clean
checksum file for new sa...
Jon Ambler
09:06 AM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #77 (Resolved): Testing pipeline on subset of data
Strandedness is correct, pipeline ready for full dataset. Jon Ambler

05/09/2019

04:29 PM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #79 (In Progress): Uploading new data from BGI
Uploading the data directly to ilifu from the BGI ftp server. Jon Ambler
04:28 PM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #79 (Resolved): Uploading new data from BGI
New samples on the BGI server need to be uploaded. Login details contained in an email.
Jon Ambler
04:26 PM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #77 (In Progress): Testing pipeline on subset of data
After dealing with some java issues, the pipeline ran through successfully.
The QC results look mostly good, just...
Jon Ambler
03:12 PM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #78 (Resolved): Proteomics raw data uploaded to Blackburn Lab researchdata
Files uploaded to:
/mnt/researchdata/BLACKBURNLAB/2019/Collaborations/Thys/keloid
Thys Potgieter

05/03/2019

09:55 AM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #77 (Resolved): Testing pipeline on subset of data
We are going to test the pipeline on a subset of the samples to make sure that the pipeline is functioning correctly.... Jon Ambler
09:40 AM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #73 (Closed): Initial meeting
Jon Ambler
09:39 AM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #74 (Resolved): Uploading of data
All samples uploaded
1 sample failed checksum
Clean/GSH-FKN-002/CL100118345_L2_WHHUMyhwEACYRAA-588_2.fq.gz
re-upl...
Jon Ambler

05/02/2019

07:32 AM Pathogen outbreak study - Pseudomonas single isolate WGS Feedback on pipeline results from Nicol group
We have been analysing the data you sent us and its looking really good. We are trying to do some additional analyses... Katie Lennard

04/29/2019

06:01 PM HIV latency transcriptomics of resting CD4+ T cells Results from run 1 sent to Walter - QC issues detected
The multiqc report highlighted potential issues with sample and/or library prep. A large proportion of reads were < 1... Katie Lennard
06:00 PM HIV latency transcriptomics of resting CD4+ T cells Run_1_merged_gene_counts.txt
Katie Lennard
09:44 AM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #74: Uploading of data
Started upload of the data to Ilifu.
Location:
/ceph/cbio/users/jambler
Jon Ambler

04/26/2019

10:52 AM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #74: Uploading of data
Ok the private folder is there on Ilifu now. Gerrit Botha

04/25/2019

05:28 PM Infant stool metaproteome analysis Support #75 (Resolved): Taxonomic profiling using BLAST of identified proteins and peptides
BLAST analysis of infant stool peptides and proteins, using the CHPC peptide BLAST pipeline. Thys Potgieter
04:57 PM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #73 (Resolved): Initial meeting
Relebohile outlined the study design, and elaborated on the research questions they are seeking to answer.
We outlin...
Jon Ambler
04:45 PM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #74: Uploading of data

I've requested to setup a POSIX group for this project (`cbio-002`) on Ilifu.
Jon for now you can move the data ...
Gerrit Botha
04:39 PM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #74 (Resolved): Uploading of data
Getting data onto Ilifu Gerrit Botha

04/24/2019

12:25 PM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) BST_bioinformatics_service_questionnaire_RM.docx
Support request form Jon Ambler
11:42 AM Identification of biomarkers in keloids and folliculitis keloidalis nuchae (FKN) Support #73 (Closed): Initial meeting
A meeting has been arranged for the 25/04/19 at 15:00 to discuss the project.
Jon Ambler
 

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